JUP - Antibodies - The Human Protein Atlas

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JUP

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA032047

Antibody CAB002139

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA032047

NCL-G-CAT

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA032047, RRID:AB_2674124

VALIDATION SUMMARY

Antibody HPA032047

Antibody CAB002139

Immunocytochemistry

Uncertain

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA032047

Antibody CAB002139

Standard validation

Uncertain

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to cell junctions & vesicles.

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

Antibody dilution

1:39

1:6

Literature conformity

The subcellular location is not consistent with literature.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA032047

Antibody CAB002139

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 54 cell types

RNA: detected in 37 tissues
Protein: detected in 38 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:25

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA032047

Antibody CAB002139

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

81.7, 32.6, 32.4, 30.3, 25.7, 11.4, 9.7

81.7, 32.6, 32.4, 30.3, 25.7, 11.4, 9.7, 0.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA032047

Antibody CAB002139

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA032047

Antibody CAB002139

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

VMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQ
YTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGV

Matching transcripts

JUP-001 - ENSP00000311113 [100%]
JUP-002 - ENSP00000394146 [100%]
JUP-003 - ENSP00000401034 [100%]
JUP-004 - ENSP00000377507 [100%]
JUP-006 - ENSP00000389886 [100%]
JUP-007 - ENSP00000411449 [100%]
JUP-008 - ENSP00000377508 [100%]
JUP-012 - ENSP00000468347 [100%]
JUP-009 - ENSP00000409948 [92%]

Other gene match

ANTIGEN VIEW

JUP-001	JUP-002	JUP-003	JUP-004	JUP-006	JUP-007	JUP-008	JUP-009	JUP-012

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.