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Immunofluorescent staining of human cell line HeLa shows localization to nucleus & nucleoli.
Antibody dilution
1:25
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038680
Antibody HPA039037
Standard validation
Approved
Figure description
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038680
Antibody HPA039037
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401514)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.6, 21.8
95.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA038680
Antibody HPA039037
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
