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ANTIBODY INFORMATION
 
 

Antibody HPA002265

  

Antibody CAB017621

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002265   sc-69750  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   4  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA002265, RRID:AB_1078142   Santa Cruz Biotechnology Cat#sc-69750, RRID:AB_1118894  

VALIDATION SUMMARY
 
 

Antibody HPA002265

  

Antibody CAB017621

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002265

  

Antibody CAB017621

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human stomach shows distinct positivity in blood plasma.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 28 cell types		   RNA: detected in 37 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002265

  

Antibody CAB017621

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 163.3   163.3, 18.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002265

  

Antibody CAB017621

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002265

  

Antibody CAB017621

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 146    

Antigen sequence

 
SVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDDEDCINRHNVYING
ITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEG
LRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNS
  

											 
																				

																		

										
											
Matching transcripts

												
																					A2M-001 - ENSP00000323929 [99%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
A2M-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002265

												
										 
																				
											
											
											
Antibody CAB017621

												
										 
																		

																	

										
											
1.

												
																						Classification of protein profiles from antibody microarrays using heat and detergent treatment

	Häggmark A et al

	N Biotechnol 2012;29(5):564-70

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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