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ANTIBODY INFORMATION
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA045725   AB5603  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 12   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045725, RRID:AB_2679431    

VALIDATION SUMMARY
 
 

Antibody HPA045725

  

Antibody CAB010648

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.    

Antibody dilution

 1:50    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human nasopharynx shows nuclear positivity in respiratory epithelial cells.  

Expression

   RNA: detected in 10 tissues
Protein: detected in 25 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:6000  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.3   34.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA045725

  

Antibody CAB010648

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 72    

Antigen sequence

 
GSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMN
GSPTYSMSYSQQGTPGMALGSM
  

											 
																				

																		

										
											
Matching transcripts

												
																					SOX2-001 - ENSP00000323588 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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