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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:66
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA022864
Antibody HPA066717
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Expression
RNA: detected in 17 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA022864
Antibody HPA066717
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415191)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
