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ANTIBODY INFORMATION
 
 

Antibody HPA056308

  

Antibody HPA066234

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA056308   HPA066234  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA056308, RRID:AB_2683095   Atlas Antibodies Cat#HPA066234, RRID:AB_2685640  

VALIDATION SUMMARY
 
 

Antibody HPA056308

  

Antibody HPA066234

  
Immunocytochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA056308

  

Antibody HPA066234

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.  

Antibody dilution

 1:30   1:135  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA056308

  

Antibody HPA066234

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 192.2, 188.2, 186.2, 139.1   192.2  

Antibody dilution

 1:150   1:450  

PROTEIN ARRAY
 
 

Antibody HPA056308

  

Antibody HPA066234

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:5400  

ANTIGEN INFORMATION
 
 

Antibody HPA056308

  

Antibody HPA066234

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 136   101  

Antigen sequence

 
NINGASYLRLPWVNPYMEGATPAIYPFLDSPNKYSLNMYKALLPQQSYSL
AQPLYSPVCTNGERFLYLPPPHYVGPHIPSSLASPMRLSTPSASPAIPPL
VHCADKSLPWKMGVSPGNPVDSHAYPHIQNSKQPRV
  
LRVDRKRKVSGDSSHTETTAEEVPEDPLLKAKRRRVSKDDWPEREMTNSS
SNHLEDPHYSELTNLKVCIELTGLHPKKQRHLLHLRERWEQQVSAADGKP
G
 

Matching transcripts

 BCOR-003 - ENSP00000367705 [100%]
BCOR-004 - ENSP00000367716 [100%]
BCOR-006 - ENSP00000384485 [100%]
BCOR-007 - ENSP00000380512 [100%]
BCOR-201 - ENSP00000345923 [100%]
  BCOR-003 - ENSP00000367705 [100%]
 

Other gene match

    
ANTIGEN VIEW
BCOR-003
BCOR-004
BCOR-006
BCOR-007
BCOR-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.