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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli.
Antibody dilution
1:6
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA058975
Standard validation
Approved
Figure description
Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells and distinct staining in vascular smooth muscle and basement membrane.
Expression
RNA: detected in 36 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA058975
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
21.6
Antibody dilution
1:80
PROTEIN ARRAY
Antibody HPA058975
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
