Fields »
ABOUT
HELP
BLOG
KRT5

ANTIBODY INFORMATION
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Lab Vision/NeoMarkers
  

Product name

 HPA059479   M7237   MS-1896  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Not known  

Other gene match

      

Released in version

 12   1   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA059479, RRID:AB_2684034   Dako Cat#M7237, RRID:AB_2281083    

VALIDATION SUMMARY
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.   Immunohistochemical staining of human esophagus shows cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 18 cell types		   RNA: detected in 23 tissues
Protein: detected in 16 cell types		   RNA: detected in 23 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9  

Antibody dilution

 1:25   1:1000   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62.4, 14.3, 12.2   62.4, 23, 21.9, 20.7, 14.3, 12.2   62.4, 23, 21.9, 20.7, 14.3, 12.2  

Antibody dilution

 1:80   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:650      

ANTIGEN INFORMATION
 
 

Antibody HPA059479

  

Antibody CAB000027

  

Antibody CAB000129

  

Antigen

 Recombinant protein fragment   Not known   Not known  

Length (aa)

 30      

Antigen sequence

 
VSFRSGGSRSFSTASAITPSVSRTSFTSVS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					KRT5-001 - ENSP00000252242 [100%] 
KRT5-003 - ENSP00000449651 [100%] 
KRT5-004 - ENSP00000448041 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KRT5-001
KRT5-003
KRT5-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.