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KRT14

ANTIBODY INFORMATION
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA000452   HPA000539   HPA023040   MS -115  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 KRT17 - ENSG00000128422 [100%]
   KRT17 - ENSG00000128422 [100%]
KRT16 - ENSG00000186832 [82%]
      

Released in version

 1   1   5   1  

References

        

Proper citation

 Atlas Antibodies Cat#HPA000452, RRID:AB_1079177     Atlas Antibodies Cat#HPA023040, RRID:AB_1852201    

VALIDATION SUMMARY
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  
Immunocytochemistry

  Approved   Approved    
Immunohistochemistry

Supported    

Validated
  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Approved   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Standard validation

   Approved   Approved    

       

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.   Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.    

Antibody dilution

   1:122   1:96    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 34 tissues.  
 

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human skin shows distinct positivity in keratinocytes.     Immunohistochemical staining of human oral mucosa shows strong positivity in basal squamous epithelial cells.   Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.  

Expression

 RNA: detected in 13 tissues
Protein: detected in 35 cell types		     RNA: detected in 13 tissues
Protein: detected in 14 cell types		   RNA: detected in 13 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH9  

Antibody dilution

 1:200     1:1600   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 51.6, 48.1, 40.3, 24.9   51.6, 51.3, 48.1, 40.3   51.6   51.6  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000   1:1000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA000452

  

Antibody HPA000539

  

Antibody HPA023040

  

Antibody CAB000134

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 125   156   59    

Antigen sequence

 
ARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQ
ALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMN
ALRGQVGGEINVEMDAAPGVDLSRI
  
LRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNA
LRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSK
TEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNL
AETENR
  
TYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVST
HEQVLRTKN
  

											 
																				

																		

										
											
Matching transcripts

												
																					KRT14-001 - ENSP00000167586 [88%] 

											 
																						KRT14-001 - ENSP00000167586 [92%] 

											 
																						KRT14-001 - ENSP00000167586 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					KRT17 - ENSG00000128422 [100%]

											 
																						KRT17 - ENSG00000128422 [100%]
KRT16 - ENSG00000186832 [82%]

											 
																						
											 
																						
											 
																				

																		

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