KRT17 - Antibodies - The Human Protein Atlas

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KRT17

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Product name

HPA000452

HPA000453

HPA000539

HPA045062

M7046

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

KRT14 - ENSG00000186847 [88%]

KRT16 - ENSG00000186832 [82%]
KRT14 - ENSG00000186847 [92%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA000452, RRID:AB_1079177

Atlas Antibodies Cat#HPA000453, RRID:AB_1079176

Atlas Antibodies Cat#HPA045062, RRID:AB_2679204

Dako Cat#M7046, RRID:AB_2133155

VALIDATION SUMMARY

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA045062.

Validated

Antibody staining overlaps with antibody HPA000453.

Standard validation

Supported

Approved

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.

Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.

Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.

Antibody dilution

1:61

1:122

1:22

Literature conformity

The subcellular location is supported by literature.

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human skin shows distinct positivity in keratinocytes.

Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.

Immunohistochemical staining of human breast shows strong cytoplasmic positivity in myoepithelial cells.

Immunohistochemical staining of human urinary bladder shows strong cytoplasmic/membranous positivity in basal layer of urothelial cells.

Expression

RNA: detected in 23 tissues
Protein: detected in 35 cell types

RNA: detected in 23 tissues
Protein: detected in 32 cell types

RNA: detected in 23 tissues
Protein: detected in 15 cell types

RNA: detected in 23 tissues
Protein: detected in 21 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:300

1:10

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

51.6, 48.1, 40.3, 24.9

48.1, 40.3

51.6, 51.3, 48.1, 40.3

48.1, 24.9

48.1, 40.3, 24.9, 11, 5.5

Antibody dilution

1:250

1:600

PROTEIN ARRAY

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:500

ANTIGEN INFORMATION

Antibody HPA000452

Antibody HPA000453

Antibody HPA000539

Antibody HPA045062

Antibody CAB000029

Antigen

Recombinant protein fragment

Native protein

Length (aa)

125

156

Antigen sequence

ARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQ
ALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMN
ALRGQVGGEINVEMDAAPGVDLSRI

TMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQ
LRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTT
RQVRTIVEEVQDGKVISSREQVHQT

LRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNA
LRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSK
TEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNL
AETENR