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LIN28B

ANTIBODY INFORMATION
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036630   HPA061745  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036630, RRID:AB_10669911   Atlas Antibodies Cat#HPA061745, RRID:AB_2684601  

VALIDATION SUMMARY
 
 

Antibody HPA036630

  

Antibody HPA061745

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated
  

Validated

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, nucleoli & cytosol.  

Antibody dilution

   1:78  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA061745 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA036630 across 72 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 2 cell types		   RNA: detected in 2 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 27.1   27.1  

Antibody dilution

 1:250   1:260  

PROTEIN ARRAY
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3100  

ANTIGEN INFORMATION
 
 

Antibody HPA036630

  

Antibody HPA061745

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 60   43  

Antigen sequence

 
KKCHYCQSIMHMVANCPHKNVAQPPASSQGRQEAESQPCTSTLPREVGGG
HGCTSPPFPQ
  
QEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT
 

Matching transcripts

 LIN28B-001 - ENSP00000344401 [100%]
  LIN28B-001 - ENSP00000344401 [100%]
 

Other gene match

    
ANTIGEN VIEW
LIN28B-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.