NOC2L - Antibodies - The Human Protein Atlas

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NOC2L

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA044258

Antibody HPA062195

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA044258

HPA062195

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA044258, RRID:AB_10961284

Atlas Antibodies Cat#HPA062195, RRID:AB_2684707

VALIDATION SUMMARY

Antibody HPA044258

Antibody HPA062195

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA044258

Antibody HPA062195

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:51

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.

Antibody dilution

1:51

1:64

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA044258

Antibody HPA062195

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA044258

Antibody HPA062195

Formal validation: Genetic

Validated

Downregulation visible in one of two siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

84.9

Loading control

Total protein image

Antibody dilution

1:114

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

84.9

Antibody dilution

1:250

1:210

PROTEIN ARRAY

Antibody HPA044258

Antibody HPA062195

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2550

ANTIGEN INFORMATION

Antibody HPA044258

Antibody HPA062195

Antigen

Recombinant protein fragment

Length (aa)

101

Antigen sequence

LTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRK
GRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDS

LLGKVQENSAYICSRRQRVSFGVSEQQAVEAWEKLTREEGTPLTLYYSHW
RKLRDREIQLEISGKERLEDLNFPEIKRRKMADRKDEDRKQFKDLFDLNS
S

Matching transcripts

NOC2L-001 - ENSP00000317992 [100%]

Other gene match

ANTIGEN VIEW

NOC2L-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.