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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003506
Antibody CAB005873
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419515)
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.9, 24, 18.3
24.9, 18.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003506
Antibody CAB005873
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
