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AFAP1

ANTIBODY INFORMATION
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA015642   AF4558  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA015642, RRID:AB_1844632    

VALIDATION SUMMARY
 
 

Antibody HPA015642

  

Antibody CAB024712

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, actin filaments & focal adhesion sites.    

Antibody dilution

 1:9    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 72 cell types		   RNA: detected in 33 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:30  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 80.7   89.8, 80.7, 9.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA015642

  

Antibody CAB024712

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 121    

Antigen sequence

 
PEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPS
GTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKR
TGSNAAQYKYGKNRVEADAKR
  

											 
																				

																		

										
											
Matching transcripts

												
																					AFAP1-001 - ENSP00000353402 [100%] 
AFAP1-201 - ENSP00000351245 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AFAP1-001
AFAP1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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