Fields »
ABOUT
HELP
BLOG
HSH2D

ANTIBODY INFORMATION
 
 

Antibody HPA036616

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036616  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 7  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA036616

  
Immunocytochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036616

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & focal adhesion sites.  

Antibody dilution

 1:102  

Literature conformity

 The subcellular location is not consistent with literature.  

WESTERN BLOT
 
 

Antibody HPA036616

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 39  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA036616

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036616

  

Antigen

 Recombinant protein fragment  

Length (aa)

 78  

Antigen sequence

 
RITSKEATSSCPPKSPLGETRQKLWRSLKMLPERGQRVRQQLKSHLATVN
LSSLLDVRRSTVISGPGTGKGSQDHSGD
 

Matching transcripts

 HSH2D-001 - ENSP00000483354 [99%]
HSH2D-009 - ENSP00000482604 [99%]
 

Other gene match

  
ANTIGEN VIEW
HSH2D-001
HSH2D-009
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.