Fields »
ABOUT
HELP
BLOG
ARID5A

ANTIBODY INFORMATION
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002860   HPA023879  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   5  

References

    

Proper citation

   Atlas Antibodies Cat#HPA023879, RRID:AB_1845014  

VALIDATION SUMMARY
 
 

Antibody HPA002860

  

Antibody HPA023879

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.  

Antibody dilution

 1:38   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1200  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.1, 56.7   64.1, 56.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA002860

  

Antibody HPA023879

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 112   115  

Antigen sequence

 
KGLTENSRHRLTPQEGLQAPGGSLREEAQAGPCPAAPIFKGCFYTHPTEV
LKPVSQHPRDFFSRLKDGVLLGPPGKEGLSVKEPQLVWGGDANRPSAFHK
GGSRKGILYPKP
  
LVLPYVRHLKGEDDKPLPTSKPRKQYKMAKENRGDDGATERPKKAKEERR
MDQMMPGKTKADAADPAPLPSQEPPRNSTEQQGLASGSSVSFVGASGCPE
AYKRLLSSFYCKGTH
 

Matching transcripts

 ARID5A-001 - ENSP00000350078 [100%]
ARID5A-004 - ENSP00000400785 [100%]
  ARID5A-001 - ENSP00000350078 [100%]
ARID5A-004 - ENSP00000400785 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARID5A-001
ARID5A-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.