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ANTIBODY INFORMATION
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   AbD Serotec
  

Product name

 HPA000368   HPA001016   HPA001115   HPA002925   MCA464  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

          

Released in version

 2   16   2   2   1  

References

          

Proper citation

     Atlas Antibodies Cat#HPA001115, RRID:AB_1078871   Atlas Antibodies Cat#HPA002925, RRID:AB_1078869    

VALIDATION SUMMARY
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  
Immunocytochemistry


Validated
      

Validated
   Supported  
Immunohistochemistry

   

Validated
   Supported  

Validated
  
Western blot

Supported   Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA002925.		      
Validated

Antibody staining overlaps with antibody HPA000368.		    
 

Standard validation

 Supported       Supported   Supported  

         

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.       Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & actin filaments.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments.  

Antibody dilution

 1:145       1:111   1:37  

Literature conformity

 The subcellular location is supported by literature.       The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

     Supported   Supported   Supported  

         

Figure description

     Immunohistochemical staining of human seminal vesicle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human seminal vesicle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human seminal vesicle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 29 cell types		   RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 26 cell types		  

Retrieval

     HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

     1:100   1:500   1:150  

Literature conformity

     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

     Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  
 

Standard validation

   Approved        

         

Figure description

   Synaptic staining in many regions of the brain.        

WESTERN BLOT
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

         

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 280.7, 280, 278.2, 245.8, 25   280.7, 280, 278.2, 245.8, 25   280.7, 280, 278.2, 276.5   280.7, 280, 278.2, 245.8, 25   280.7, 280, 278.2, 276.5, 245.8, 29.9, 25  

Antibody dilution

 1:500   1:500   1:500   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000   1:3000   1:3000   1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA000368

  

Antibody HPA001016

  

Antibody HPA001115

  

Antibody HPA002925

  

Antibody CAB000356

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 119   119   113   149    

Antigen sequence

 
FQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDV
TSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPS
EAGLHEMDIRYDNMHIPGS
  
FQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDV
TSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPS
EAGLHEMDIRYDNMHIPGS
  
IKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIP
EISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYK
GQHVPGSPFQFTV
  
VICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQT
WAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTI
TDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVT
  

											 
																				

																		

										
											
Matching transcripts

												
																					FLNA-001 - ENSP00000353467 [100%] 
FLNA-002 - ENSP00000358866 [100%] 
FLNA-015 - ENSP00000398215 [100%] 
FLNA-022 - ENSP00000358872 [100%] 
FLNA-201 - ENSP00000416926 [100%] 
FLNA-202 - ENSP00000480593 [100%] 

											 
																						FLNA-001 - ENSP00000353467 [100%] 
FLNA-002 - ENSP00000358866 [100%] 
FLNA-015 - ENSP00000398215 [100%] 
FLNA-022 - ENSP00000358872 [100%] 
FLNA-201 - ENSP00000416926 [100%] 
FLNA-202 - ENSP00000480593 [100%] 

											 
																						FLNA-001 - ENSP00000353467 [100%] 
FLNA-002 - ENSP00000358866 [100%] 
FLNA-010 - ENSP00000358863 [100%] 
FLNA-022 - ENSP00000358872 [100%] 
FLNA-201 - ENSP00000416926 [100%] 

											 
																						FLNA-001 - ENSP00000353467 [100%] 
FLNA-002 - ENSP00000358866 [100%] 
FLNA-015 - ENSP00000398215 [100%] 
FLNA-022 - ENSP00000358872 [100%] 
FLNA-201 - ENSP00000416926 [100%] 
FLNA-202 - ENSP00000480593 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FLNA-001
FLNA-002
FLNA-010
FLNA-015
FLNA-022
FLNA-201
FLNA-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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