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Immunohistochemical staining of human placenta shows cytoplasmic positivity in stromal cells.
Immunohistochemical staining of human thyroid gland shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 31 tissues Protein: detected in 7 cell types
RNA: detected in 31 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA062066
Antibody CAB032904
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
104.9
104.9
Antibody dilution
1:80
1:500
PROTEIN ARRAY
Antibody HPA062066
Antibody CAB032904
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
