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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ENPP1
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ENPP1

Gene description

Ectonucleotide pyrophosphatase/phosphodiesterase 1

Protein class

CD markers
Disease related genes
Enzymes
Potential drug targets
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

1
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (LHCN-M2, U-138 MG)
HPA (normal tissue):Tissue enhanced (parathyroid gland, placenta)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Membranous expression in a subset of cells including placental stroma, chondrocytes, exocrine pancreas, renal tubules, thyroid and parathyroid gland.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 2 antibodies.
HPA062066 , CAB032904
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PROGNOSTIC SUMMARY

Gene product is not prognostic.
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA062066
CAB032904

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A few cases of hepatocellular carcinomas, melanomas, testicular, pancreatic and thyroid cancers displayed moderate membranous positivity. Remaining cancer tissues were generally negative.

GENE INFORMATION

Gene name

ENPP1 (HGNC Symbol)

Synonyms

M6S1, NPPS, PC-1, PCA1, PDNP1

Description

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (HGNC Symbol)

Entrez gene summary

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Chromosome

6

Cytoband

q23.2

Chromosome location (bp)

131808016 - 131895155

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000197594 (version 83.38)

Entrez gene

5167

UniProt

P22413 (UniProt - Evidence at protein level)

neXtProt

NX_P22413

Antibodypedia

ENPP1 antibodies

PROTEIN BROWSER

ENPP1-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ENPP1-001
ENSP00000354238
ENST00000360971
P22413 [Direct mapping]
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 Alkaline phosphodiesterase I Nucleotide pyrophosphatase
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Enzymes
   ENZYME proteins
   Hydrolases
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0003676 [nucleic acid binding]
GO:0003824 [catalytic activity]
GO:0004528 [phosphodiesterase I activity]
GO:0004551 [nucleotide diphosphatase activity]
GO:0005044 [scavenger receptor activity]
GO:0005158 [insulin receptor binding]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005615 [extracellular space]
GO:0005765 [lysosomal membrane]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006091 [generation of precursor metabolites and energy]
GO:0006766 [vitamin metabolic process]
GO:0006767 [water-soluble vitamin metabolic process]
GO:0006771 [riboflavin metabolic process]
GO:0006796 [phosphate-containing compound metabolic process]
GO:0006898 [receptor-mediated endocytosis]
GO:0006955 [immune response]
GO:0008152 [metabolic process]
GO:0008270 [zinc ion binding]
GO:0009143 [nucleoside triphosphate catabolic process]
GO:0009986 [cell surface]
GO:0016021 [integral component of membrane]
GO:0016323 [basolateral plasma membrane]
GO:0016787 [hydrolase activity]
GO:0030247 [polysaccharide binding]
GO:0030308 [negative regulation of cell growth]
GO:0030500 [regulation of bone mineralization]
GO:0030505 [inorganic diphosphate transport]
GO:0030643 [cellular phosphate ion homeostasis]
GO:0030730 [sequestering of triglyceride]
GO:0031214 [biomineral tissue development]
GO:0031953 [negative regulation of protein autophosphorylation]
GO:0032869 [cellular response to insulin stimulus]
GO:0035529 [NADH pyrophosphatase activity]
GO:0042803 [protein homodimerization activity]
GO:0044281 [small molecule metabolic process]
GO:0045599 [negative regulation of fat cell differentiation]
GO:0045719 [negative regulation of glycogen biosynthetic process]
GO:0046325 [negative regulation of glucose import]
GO:0046627 [negative regulation of insulin receptor signaling pathway]
GO:0046872 [metal ion binding]
GO:0047429 [nucleoside-triphosphate diphosphatase activity]
GO:0050427 [3'-phosphoadenosine 5'-phosphosulfate metabolic process]
GO:0050656 [3'-phosphoadenosine 5'-phosphosulfate binding]
GO:0090305 [nucleic acid phosphodiester bond hydrolysis]
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925 aa
104.9 kDa
No 1

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