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TLK1

ANTIBODY INFORMATION
 
 

Antibody HPA016043

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016043  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA016043, RRID:AB_1858027  

VALIDATION SUMMARY
 
 

Antibody HPA016043

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot


Validated
  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016043

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.  

Antibody dilution

 1:80  

Literature conformity

 The subcellular location is supported by literature.  
 

Validation: Genetic

 Supported

Significant downregulation 10-25% by one/two siRNA:s		  

 

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:80  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016043

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear and slightly weaker cytoplasmic positivity in tubules and glomeruli.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016043

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 89, 86.7, 81.9, 77.1, 17.7  

Loading control

 Total protein image  

Antibody dilution

 1:178  
 

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402184)		  

Target mass (kDa)

 89, 86.7, 81.9, 77.1, 17.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA016043

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA016043

  

Antigen

 Recombinant protein fragment  

Length (aa)

 112  

Antigen sequence

 
ETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSS
PVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSF
KIIQTDLTMLKL
 

Matching transcripts

 TLK1-001 - ENSP00000411099 [100%]
TLK1-012 - ENSP00000428113 [100%]
TLK1-005 - ENSP00000409222 [99%]
TLK1-002 - ENSP00000354089 [84%]
TLK1-010 - ENSP00000393165 [83%]
 

Other gene match

  
ANTIGEN VIEW
TLK1-001
TLK1-002
TLK1-005
TLK1-010
TLK1-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.