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CD3E

ANTIBODY INFORMATION
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040957   HPA043955   M7254   AMAb90876   AMAb90879  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 13   13   1   14   14  

References

          

Proper citation

 Atlas Antibodies Cat#HPA040957, RRID:AB_2677222   Atlas Antibodies Cat#HPA043955, RRID:AB_2678747     Atlas Antibodies Cat#AMAb90876, RRID:AB_2665707   Atlas Antibodies Cat#AMAb90879, RRID:AB_2665708  

VALIDATION SUMMARY
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  
Immunohistochemistry

Supported  

Validated
  

Validated
   Supported  

Validated
  
Western blot

Supported   Supported   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Approved        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
   
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphiod tissue.   Immunohistochemical staining of human tonsil shows strong cytplasmic positivity in lymphoid cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.   Immunohistochemical staining of human tonsil shows cytoplasmic positivity in non-germinal center cells.   Immunohistochemical staining of human tonsil shows cytoplasmic positivity in T-cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 8 cell types		   RNA: detected in 34 tissues
Protein: detected in 37 cell types		   RNA: detected in 34 tissues
Protein: detected in 6 cell types		   RNA: detected in 34 tissues
Protein: detected in 14 cell types		   RNA: detected in 34 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:800   1:100   1:750   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 23.1, 22.6   23.1, 22.6   23.1, 22.6   23.1, 22.6   23.1, 22.6  

Antibody dilution

 1:250   1:250   1:500   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:6000        

ANTIGEN INFORMATION
 
 

Antibody HPA040957

  

Antibody HPA043955

  

Antibody CAB000010

  

Antibody CAB072863

  

Antibody CAB072864

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein   Synthetic peptide   Synthetic peptide  

Length (aa)

 102   57        

Antigen sequence

 
NEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKN
IGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCME
MD
  
WSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYS
GLNQRRI
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD3E-001 - ENSP00000354566 [100%] 
CD3E-007 - ENSP00000433975 [93%] 

											 
																						CD3E-001 - ENSP00000354566 [100%] 
CD3E-007 - ENSP00000433975 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD3E-001
CD3E-007
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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