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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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CD3E
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CD3E

Gene description

CD3e molecule, epsilon (CD3-TCR complex)

Protein class

CD markers
Disease related genes
FDA approved drug targets
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enriched (MOLT-4)
HPA (normal tissue):Tissue enhanced (lymph node, tonsil)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Selective cytoplasmic expression in T-cells.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining consistent with RNA expression data.

Reliability score

Supported based on 5 antibodies.
HPA040957 , HPA043955 , CAB000010 , CAB072863 , CAB072864
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (favourable), renal cancer (unfavourable), melanoma (favourable), head and neck cancer (favourable), cervical cancer (favourable) and breast cancer (favourable).
Endometrial cancer p<0.001
Renal cancer p<0.001
Melanoma p<0.001
Head and neck cancer p<0.001
Cervical cancer p<0.001
Breast cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA040957
HPA043955
CAB000010
CAB072863
CAB072864

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

All cancer cells were negative. Immune cells showed weak to moderate cytoplasmic staining.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were generally negative. Strong cytoplasmic staining of T-cells was observed in several cancer tissues.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Strong cytoplasmic staining was displayed in a single lymphoma. Remaining cancer tissues were in general negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate cytoplasmic staining in several cases of prostate cancer. All other cancer cells were negative. Strong cytoplasmic staining in subsets of immune cells seen in most cancer tissues.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

All cancer cells were negative. Strong cytoplasmic staining in infiltrating immune cells in the stroma of most cancer tissues.

GENE INFORMATION

Gene name

CD3E

Synonyms

Description

CD3e molecule, epsilon (CD3-TCR complex) (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Chromosome

11

Cytoband

q23.3

Chromosome location (bp)

118304545 - 118316175

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000198851 (version 83.38)

Entrez gene

916

UniProt

P07766 (UniProt - Evidence at protein level)

neXtProt

NX_P07766

Antibodypedia

CD3E antibodies

PROTEIN BROWSER

CD3E-001
CD3E-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CD3E-001
ENSP00000354566
ENST00000361763
P07766 [Direct mapping]
T-cell surface glycoprotein CD3 epsilon chain
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CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Disease related genes
FDA approved drug targets
   Biotech drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001772 [immunological synapse]
GO:0002669 [positive regulation of T cell anergy]
GO:0004871 [signal transducer activity]
GO:0004888 [transmembrane signaling receptor activity]
GO:0005057 [receptor signaling protein activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0005911 [cell-cell junction]
GO:0006461 [protein complex assembly]
GO:0007166 [cell surface receptor signaling pathway]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0007172 [signal complex assembly]
GO:0007186 [G-protein coupled receptor signaling pathway]
GO:0007584 [response to nutrient]
GO:0009897 [external side of plasma membrane]
GO:0010628 [positive regulation of gene expression]
GO:0010629 [negative regulation of gene expression]
GO:0016020 [membrane]
GO:0017124 [SH3 domain binding]
GO:0019901 [protein kinase binding]
GO:0030159 [receptor signaling complex scaffold activity]
GO:0031295 [T cell costimulation]
GO:0032729 [positive regulation of interferon-gamma production]
GO:0032753 [positive regulation of interleukin-4 production]
GO:0033077 [T cell differentiation in thymus]
GO:0035556 [intracellular signal transduction]
GO:0042101 [T cell receptor complex]
GO:0042102 [positive regulation of T cell proliferation]
GO:0042105 [alpha-beta T cell receptor complex]
GO:0042110 [T cell activation]
GO:0042608 [T cell receptor binding]
GO:0042981 [regulation of apoptotic process]
GO:0045060 [negative thymic T cell selection]
GO:0045086 [positive regulation of interleukin-2 biosynthetic process]
GO:0045879 [negative regulation of smoothened signaling pathway]
GO:0046641 [positive regulation of alpha-beta T cell proliferation]
GO:0046649 [lymphocyte activation]
GO:0046982 [protein heterodimerization activity]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0050776 [regulation of immune response]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0050852 [T cell receptor signaling pathway]
GO:0050870 [positive regulation of T cell activation]
GO:0097190 [apoptotic signaling pathway]
Show all
207 aa
23.1 kDa
Yes 1
CD3E-007
ENSP00000433975
ENST00000528600
E9PSH8 [Direct mapping]
T-cell surface glycoprotein CD3 epsilon chain
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004888 [transmembrane signaling receptor activity]
GO:0005515 [protein binding]
GO:0007166 [cell surface receptor signaling pathway]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
Show all
201 aa
22.6 kDa
Yes 1

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The Human Protein Atlas project is funded
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