NELFE - Antibodies - The Human Protein Atlas

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NELFE

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA007187

HPA007594

HPA046502

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA007187, RRID:AB_1856161

Atlas Antibodies Cat#HPA007594, RRID:AB_2667867

Atlas Antibodies Cat#HPA046502, RRID:AB_2679677

VALIDATION SUMMARY

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA046502.

Validated

Antibody staining overlaps with antibody HPA007594.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:34

1:179

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 62 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

43.9, 43.2, 40.2, 38.2, 33.7, 30.1, 17.3

43.9, 43.2, 40.2

Antibody dilution

1:250

1:890

PROTEIN ARRAY

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6000

1:7100

ANTIGEN INFORMATION

Antibody HPA007187

Antibody HPA007594

Antibody HPA046502

Antigen

Recombinant protein fragment

Length (aa)

122

109

Antigen sequence

LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIK
AETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQ
RKSLYESFVSSSDRLRELGPDG

LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIK
AETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQ
RKSLYESFVSSSDRLRELGPDG

VYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAEL
NGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQI
VYSDDVYKE

Matching transcripts

NELFE-001 - ENSP00000364578 [100%]
NELFE-009 - ENSP00000394340 [100%]
NELFE-011 - ENSP00000409389 [100%]
NELFE-013 - ENSP00000364574 [100%]
NELFE-007 - ENSP00000397914 [96%]
NELFE-010 - ENSP00000414029 [96%]
NELFE-012 - ENSP00000388400 [89%]

NELFE-001 - ENSP00000364578 [100%]
NELFE-012 - ENSP00000388400 [100%]
NELFE-013 - ENSP00000364574 [100%]

Other gene match

ANTIGEN VIEW

NELFE-001	NELFE-007	NELFE-009	NELFE-010	NELFE-011	NELFE-012	NELFE-013

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.