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Gene name

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Method

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Method

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Antibodies

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CSHL1
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA043715

 

Antibody CAB025646

 

Provider

Atlas Antibodies
Sigma-Aldrich
  R&D Systems
 

Product name

HPA043715   MAB1067  

Host species

Rabbit   Mouse  

Clonality

pAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

GH2 - ENSG00000136487 [95%]
CSH1 - ENSG00000136488 [100%]
CSH2 - ENSG00000213218 [100%]
GH1 - ENSG00000259384 [95%]
   

Released in version

10   6  

References

   

Proper citation

Atlas Antibodies Cat#HPA043715, RRID:AB_2678632    

VALIDATION SUMMARY

 
 

Antibody HPA043715

 

Antibody CAB025646

 
Immunohistochemistry

Supported  

Validated
 
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA043715

 

Antibody CAB025646

 

Formal validation: Orthogonal

 
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.
 

 
 

Figure description

  Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

Supported   Supported  

   

Figure description

Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in cells in anterior lobe.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

RNA: detected in 1 tissues
Protein: detected in 2 cell types
  RNA: detected in 1 tissues
Protein: detected in 1 cell types
 

Retrieval

HIER pH6   HIER pH6  

Antibody dilution

1:2000   1:2000  

Literature conformity

Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA043715

 

Antibody CAB025646

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
  Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
   

Target mass (kDa)

25.5, 25.4, 25, 24.9, 24.8, 23.1, 23, 20.4, 20.2, 18.4, 18.2, 15.9, 15, 14.9, 14, 13.9   25.4, 24.9, 19.6, 18.6, 18.2, 15.9, 14.9  

Antibody dilution

1:250   1:500  

PROTEIN ARRAY

 
 

Antibody HPA043715

 

Antibody CAB025646

 

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
   

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:6000    

ANTIGEN INFORMATION

 
 

Antibody HPA043715

 

Antibody CAB025646

 

Antigen

Recombinant protein fragment   Recombinant protein  

Length (aa)

42    

Antigen sequence

SHNHDALLKNYGLLYCFRKDMDKVETFLRMVQCRSVEGSCGF
 
 

Matching transcripts

CSHL1-001 - ENSP00000316360 [98%]
CSHL1-002 - ENSP00000259003 [98%]
CSHL1-008 - ENSP00000402632 [98%]
CSHL1-009 - ENSP00000309524 [98%]
CSHL1-201 - ENSP00000376569 [98%]
CSHL1-202 - ENSP00000483369 [98%]
   

Other gene match

GH2 - ENSG00000136487 [95%]
CSH1 - ENSG00000136488 [100%]
CSH2 - ENSG00000213218 [100%]
GH1 - ENSG00000259384 [95%]
   
ANTIGEN VIEW
CSHL1-001
CSHL1-002
CSHL1-008
CSHL1-009
CSHL1-201
CSHL1-202
 

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