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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA043715

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA043715  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

GH2 - ENSG00000136487 [95%]
CSH1 - ENSG00000136488 [100%]
CSHL1 - ENSG00000204414 [98%]
GH1 - ENSG00000259384 [95%]
 

Released in version

10  

References

 

Proper citation

Atlas Antibodies Cat#HPA043715, RRID:AB_2678632  

VALIDATION SUMMARY

 
 

Antibody HPA043715

 
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA043715

 

Standard validation

Supported  

 

Figure description

Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in cells in anterior lobe.  

Expression

RNA: detected in 6 tissues
Protein: detected in 2 cell types
 

Retrieval

HIER pH6  

Antibody dilution

1:2000  

Literature conformity

Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA043715

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
 

 

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

25.5, 25.4, 25, 24.9, 24.8, 23.1, 23, 20.4, 20.2, 18.4, 18.2, 15.9, 15, 14.9, 14, 13.9  

Antibody dilution

1:250  

PROTEIN ARRAY

 
 

Antibody HPA043715

 

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:6000  

ANTIGEN INFORMATION

 
 

Antibody HPA043715

 

Antigen

Recombinant protein fragment  

Length (aa)

42  

Antigen sequence

SHNHDALLKNYGLLYCFRKDMDKVETFLRMVQCRSVEGSCGF
 

Matching transcripts

CSH2-001 - ENSP00000376623 [100%]
CSH2-002 - ENSP00000453452 [100%]
CSH2-201 - ENSP00000308396 [100%]
CSH2-202 - ENSP00000478842 [100%]
CSH2-203 - ENSP00000480793 [100%]
 

Other gene match

GH2 - ENSG00000136487 [95%]
CSH1 - ENSG00000136488 [100%]
CSHL1 - ENSG00000204414 [98%]
GH1 - ENSG00000259384 [95%]
 
ANTIGEN VIEW
CSH2-001
CSH2-002
CSH2-201
CSH2-202
CSH2-203
 

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