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ANTIBODY INFORMATION
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001231   sc-7274  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 3   2  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA001231, RRID:AB_1079286   Santa Cruz Biotechnology Cat#sc-7274, RRID:AB_627897  

VALIDATION SUMMARY
 
 

Antibody HPA001231

  

Antibody CAB004492

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.    

Antibody dilution

 1:39    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 28 cell types		   RNA: detected in 36 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.6, 56, 17.1   58.6, 56, 17.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001231

  

Antibody CAB004492

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 141    

Antigen sequence

 
KGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRF
QTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLL
TKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPG
  

											 
																				

																		

										
											
Matching transcripts

												
																					LYN-002 - ENSP00000428924 [100%] 
LYN-006 - ENSP00000428313 [100%] 
LYN-003 - ENSP00000428424 [85%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LYN-002
LYN-003
LYN-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001231

												
										 
																				
											
											
											
Antibody CAB004492

												
										 
																		

																	

										
											
1.

												
																						Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers

	Kiflemariam S et al

	PLoS One 2012;7(3):e32927

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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