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Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Antibody dilution
1:24
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA030471
Antibody HPA030472
Antibody HPA036584
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 17 tissues Protein: detected in 76 cell types
RNA: detected in 17 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:600
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030471
Antibody HPA030472
Antibody HPA036584
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53.3, 36.9, 21.3
53.3, 36.9
53.3, 22.2, 20.2, 18.2, 18.1, 12.2
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA030471
Antibody HPA030472
Antibody HPA036584
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
