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Immunohistochemical staining of human prostate shows strong granular cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong membranous positivity in a subset of cells in tubules along with distinctly stained extracellular material/ plasma.
Expression
RNA: detected in 18 tissues Protein: detected in 47 cell types
RNA: detected in 18 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:85
1:65
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043823
Antibody CAB069427
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
104.8
104.8, 15.3, 3.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA043823
Antibody CAB069427
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
