ABLIM1 - Antibodies - The Human Protein Atlas

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ABLIM1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA038951

Antibody HPA038952

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038951

HPA038952

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA038951, RRID:AB_10673017

Atlas Antibodies Cat#HPA038952, RRID:AB_10670915

VALIDATION SUMMARY

Antibody HPA038951

Antibody HPA038952

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA038951

Antibody HPA038952

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:64

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA038951

Antibody HPA038952

Standard validation

Approved

Figure description

Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human esophagus shows strong cytoplasmic and membranous positivity in squamous epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 63 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:50

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038951

Antibody HPA038952

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

87.7, 84.6, 84.4, 81.1, 52.1, 46.1

84.6, 84.4, 52.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA038951

Antibody HPA038952

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA038951

Antibody HPA038952

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

RSSLLASRYDSPINSASHIPSSKTASLPGYGRNGLHRPVSTDFAQYNSYG
DVSGGVRDYQTLPDGHMPAMRMDR

QGSTVWHPDCKQSTKTEEKLRLPNIRRSSSDFFYSKSLIRRTGRSPSLQP
TRTSS

Matching transcripts

ABLIM1-001 - ENSP00000277895 [100%]
ABLIM1-002 - ENSP00000376682 [100%]
ABLIM1-005 - ENSP00000358260 [100%]
ABLIM1-009 - ENSP00000376679 [100%]
ABLIM1-010 - ENSP00000358257 [100%]
ABLIM1-201 - ENSP00000358256 [99%]
ABLIM1-202 - ENSP00000358270 [99%]
ABLIM1-203 - ENSP00000433629 [99%]

ABLIM1-005 - ENSP00000358260 [100%]
ABLIM1-009 - ENSP00000376679 [100%]
ABLIM1-201 - ENSP00000358256 [100%]

Other gene match

ANTIGEN VIEW

ABLIM1-001	ABLIM1-002	ABLIM1-005	ABLIM1-009	ABLIM1-010	ABLIM1-201	ABLIM1-202	ABLIM1-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.