BNIP3L - Antibodies - The Human Protein Atlas

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BNIP3L

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA015652

Antibody CAB025371

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA015652

AF4030

Host species

Rabbit

Goat

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA015652, RRID:AB_1845406

VALIDATION SUMMARY

Antibody HPA015652

Antibody CAB025371

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA015652

Antibody CAB025371

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Antibody dilution

1:67

1:62

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA015652

Antibody CAB025371

Standard validation

Supported

Figure description

Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity.

Immunohistochemical staining of human corpus, uterine shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 58 cell types

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA015652

Antibody CAB025371

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

25.3, 23.9, 20.1, 19.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA015652

Antibody CAB025371

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA015652

Antibody CAB025371

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

133

Antigen sequence

SNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHC
DSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSD
WSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMK

Matching transcripts

BNIP3L-001 - ENSP00000370003 [100%]
BNIP3L-002 - ENSP00000429698 [100%]
BNIP3L-004 - ENSP00000429851 [100%]
BNIP3L-006 - ENSP00000428597 [100%]
BNIP3L-007 - ENSP00000429171 [100%]
BNIP3L-201 - ENSP00000479522 [100%]

Other gene match

ANTIGEN VIEW

BNIP3L-001	BNIP3L-002	BNIP3L-004	BNIP3L-006	BNIP3L-007	BNIP3L-201

RELEVANT PUBLICATIONS

Antibody HPA015652

Antibody CAB025371

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

MicroRNA-137 is a novel hypoxia-responsive microRNA that inhibits mitophagy via regulation of two mitophagy receptors FUNDC1 and NIX
Li W et al
J Biol Chem 2014;289(15):10691-701
Application: ICC-IF

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.