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BNIP3L

ANTIBODY INFORMATION
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA015652   AF4030  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   5  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA015652, RRID:AB_1845406    

VALIDATION SUMMARY
 
 

Antibody HPA015652

  

Antibody CAB025371

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:67   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity.   Immunohistochemical staining of human corpus, uterine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 25.3, 23.9, 20.1, 19.6   25.3, 23.9, 20.1, 19.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA015652

  

Antibody CAB025371

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 133    

Antigen sequence

 
SNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHC
DSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSD
WSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMK
  

											 
																				

																		

										
											
Matching transcripts

												
																					BNIP3L-001 - ENSP00000370003 [100%] 
BNIP3L-002 - ENSP00000429698 [100%] 
BNIP3L-004 - ENSP00000429851 [100%] 
BNIP3L-006 - ENSP00000428597 [100%] 
BNIP3L-007 - ENSP00000429171 [100%] 
BNIP3L-201 - ENSP00000479522 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BNIP3L-001
BNIP3L-002
BNIP3L-004
BNIP3L-006
BNIP3L-007
BNIP3L-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA015652

												
										 
																				
											
											
											
Antibody CAB025371

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

																		

										
											
2.

												
																						MicroRNA-137 is a novel hypoxia-responsive microRNA that inhibits mitophagy via regulation of two mitophagy receptors FUNDC1 and NIX

	Li W et al

	J Biol Chem 2014;289(15):10691-701

			Application: ICC-IF				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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