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GORASP2

ANTIBODY INFORMATION
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035274   HPA035275  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA035274, RRID:AB_2674546   Atlas Antibodies Cat#HPA035275, RRID:AB_10602600  

VALIDATION SUMMARY
 
 

Antibody HPA035274

  

Antibody HPA035275

  
Immunocytochemistry


Validated
  

Validated

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by both siRNA:s.		  

 
 

Figure description

   Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

   1:43  
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA035275.		  
Validated

Antibody staining overlaps with antibody HPA035274.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.  

Antibody dilution

 1:23   1:43  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA035275 across 76 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA035274 across 76 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:250  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.1   47.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA035274

  

Antibody HPA035275

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 73   74  

Antigen sequence

 
SGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRV
GDSTPVSEKPVSAAVDANASESP
  
PTRPFEEGKKISLPGQMAGTPITPLKDGFTEVQLSSVNPPSLSPPGTTGI
EQSLTGLSISSTPPAVSSVLSTGV
 

Matching transcripts

 GORASP2-001 - ENSP00000234160 [100%]
  GORASP2-001 - ENSP00000234160 [100%]
 

Other gene match

    
ANTIGEN VIEW
GORASP2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.