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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytokinetic bridge.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytokinetic bridge.
Antibody dilution
1:20
1:25
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028120
Antibody HPA028258
Antibody HPA028783
Antibody CAB040294
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA028783 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA028120 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in celsl in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 27 tissues Protein: detected in 48 cell types
RNA: detected in 27 tissues Protein: detected in 44 cell types
RNA: detected in 27 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:15
1:70
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028120
Antibody HPA028258
Antibody HPA028783
Antibody CAB040294
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402655)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402655)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402655)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.3
31.3
31.3
31.3
Antibody dilution
1:125
1:125
1:125
1:500
PROTEIN ARRAY
Antibody HPA028120
Antibody HPA028258
Antibody HPA028783
Antibody CAB040294
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
