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ANTIBODY INFORMATION
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030957   HPA039983  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA030957, RRID:AB_10670149   Atlas Antibodies Cat#HPA039983, RRID:AB_2676784  

VALIDATION SUMMARY
 
 

Antibody HPA030957

  

Antibody HPA039983

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.  

Antibody dilution

   1:85  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human eye shows moderate positivity in the lens.   Immunohistochemical staining of human eye shows strong positivity in the lens.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 0 cell types		   RNA: detected in 3 tissues
Protein: detected in 0 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:2000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.3   57.3, 23.5, 21.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA030957

  

Antibody HPA039983

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 91   89  

Antigen sequence

 
IISFPALTFLNNHDQPFMQELVDGLYHTGANVESFSSSTRPGQMEISFLP
EFGISSADNAFTLRTGVKEVARKYNYIASFFIETGFCDSGI
  
VSRSKTIPAHFFQEKVSHGVCMPRGYLEVIPNPKDNEMNNIRATCFNSDI
VLMPELSTFRVLPWADRTARVICDTFTVTGEPLLTSPRY
 

Matching transcripts

 LGSN-001 - ENSP00000359691 [100%]
  LGSN-001 - ENSP00000359691 [100%]
LGSN-002 - ENSP00000431246 [89%]
LGSN-201 - ENSP00000359692 [89%]
 

Other gene match

    
ANTIGEN VIEW
LGSN-001
LGSN-002
LGSN-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.