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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in a subset of glandular cells.
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 4 tissues Protein: detected in 2 cell types
RNA: detected in 4 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:4000
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021612
Antibody CAB016729
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
17.1
17.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA021612
Antibody CAB016729
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
