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Immunohistochemical staining of human hippocampus shows nuclear and cytoplasmic positivity in subsets of glial cells.
Immunohistochemical staining of human heart muscle shows nuclear and cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 55 cell types
RNA: detected in 37 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:30
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006462
Antibody CAB002599
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401900)
Target mass (kDa)
15.9, 10.5
15.9, 10.5, 6, 3.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA006462
Antibody CAB002599
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
