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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & cytokinetic bridge.
Antibody dilution
1:89
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041183
Antibody HPA044248
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and in granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and cells in granular layer.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:500
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041183
Antibody HPA044248
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411237)
Target mass (kDa)
15.3, 14.3
15.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041183
Antibody HPA044248
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
