SFMBT1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

SFMBT1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036152

HPA036153

HPA064564

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA036152, RRID:AB_2674972

Atlas Antibodies Cat#HPA036153, RRID:AB_10669667

Atlas Antibodies Cat#HPA064564, RRID:AB_2685288

VALIDATION SUMMARY

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA064564.

Validated

Antibody staining overlaps with antibody HPA036152.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.

Antibody dilution

1:90

1:83

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human pancreas shows moderate cytoplasmic and nuclear positivity in exocrine glandular cells.

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts, Leydig cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 58 cell types

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:40

1:2000

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

98.1

Antibody dilution

1:250

1:400

PROTEIN ARRAY

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:30000

1:3350

ANTIGEN INFORMATION

Antibody HPA036152

Antibody HPA036153

Antibody HPA064564

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DIRKADLYPIGWCEQNKKTLEAPEGIRDKVSDWDEFLRQTLIGACSPPVP
LLEGLRNGRNPLDLIAPGSRLECQAFQDS

LSTWIVTVVENIGGRLKLRYEGLESSDNYEHWLYYLDPFLHHVGWAAQQG
YELQPPSAIRHLKNEAEWQEILAKVKEEE

YTHYYGKKKNKRIGRPPGGHSNLACALKKASKRRKRRKNVFVHKKKRSSA
SVDNTPAGSPQGS

Matching transcripts

SFMBT1-001 - ENSP00000378235 [100%]

Other gene match

ANTIGEN VIEW

SFMBT1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.